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barcoded pcr libraries  (Nextera AS)

 
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    Nextera AS barcoded pcr libraries
    Barcoded Pcr Libraries, supplied by Nextera AS, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/barcoded+pcr+libraries/barcoded+pcr+libraries/pm40593605-310-14-15
    Average 90 stars, based on 1 article reviews
    barcoded pcr libraries - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Sequencing:

    Article Title: Microbial bioremediation of persistent organic pollutants in plant tissues provides crop growth promoting liquid fertilizer.
    Article Snippet: The concentration of the isolated DNA was assessed via PicoGreen measurement (Quant-iTTM PicoGreenTM dsDNA Assay Kit, Thermo Fisher). .. To sequence theV3-V4 regionof thebacterial 16S rRNAgene, two-step, Nextera barcoded PCR libraries were generated using 341 F (5′- CCT ACGGGNGGCWGCAG -3′) and 805R (5′- GACTACHVGGGTATCTAA TCC -3′) with 20 PCR cycles for the first step and 10 PCR cycles for the second step. .. To sequence the internal transcribed spacer (ITS2) regions of the fungal 18S rRNA gene, two-step, Nextera barcoded PCR libraries were generated using ITS3 (5′- GCA TCG ATG AAG AAC GCA GC -3′), and ITS4 (5′- TCC TCC GCT TAT TGA TAT GC -3′) with 20 PCR cycles for the first step and 15 PCR cycles for the second step.

    Article Title: eDNA metabarcoding reveals high soil fungal diversity and variation in community composition among Spanish cliffs
    Article Snippet: The concentration of the isolated DNA was assessed with PicoGreen measurement (Quant‐iTTM PicoGreenTM dsDNA Assay Kit; Thermo Fisher). .. To sequence the internal transcribed spacer (ITS2) regions of the fungal 18 S rRNA gene, two‐step, Nextera barcoded PCR libraries using the locus‐specific primer pair ITS3 (5′‐GCA TCG ATG AAG AAC GCA GC‐3′) and ITS4 (5′‐TCC TCC GCT TAT TGA TAT GC‐3′) with 20 PCR cycles for the first step and 20 PCR cycles for the second step were created. .. Subsequently, the PCR libraries were sequenced on an Illumina MiSeq platform using a v2 500 cycles kit.

    Article Title: Drivers of wood‐inhabiting fungal diversity in European and Oriental beech forests
    Article Snippet: DNA concentrations were quantified using a NanoDrop UV–Vis spectrophotometer (Peqlab Biotechnologie GmbH, Erlangen, Germany). .. For sequencing the internal transcribed spacer (ITS2) regions of the fungal 18S rRNA gene, we applied for two‐step, Nextera barcoded PCR libraries using the locus specific primer pair ITS3 (5′‐GCA TCG ATG AAG AAC GCA GC‐3′) and ITS4 (5′‐TCC TCC GCT TAT TGA TAT GC‐3′) with 20 PCR cycles for the first step and 15 PCR cycles for the second step were created. .. Subsequently the PCR libraries were sequenced on an Illumina MiSeq platform using a v2 500 cycles kit.

    Article Title: Microbial bioremediation of persistent organic pollutants in plant tissues provides crop growth promoting liquid fertilizer.
    Article Snippet: To sequence theV3-V4 regionof thebacterial 16S rRNAgene, two-step, Nextera barcoded PCR libraries were generated using 341 F (5′- CCT ACGGGNGGCWGCAG -3′) and 805R (5′- GACTACHVGGGTATCTAA TCC -3′) with 20 PCR cycles for the first step and 10 PCR cycles for the second step. .. To sequence the internal transcribed spacer (ITS2) regions of the fungal 18S rRNA gene, two-step, Nextera barcoded PCR libraries were generated using ITS3 (5′- GCA TCG ATG AAG AAC GCA GC -3′), and ITS4 (5′- TCC TCC GCT TAT TGA TAT GC -3′) with 20 PCR cycles for the first step and 15 PCR cycles for the second step. ..

    Article Title: Microbial bioremediation of persistent organic pollutants in plant tissues provides crop growth promoting liquid fertilizer
    Article Snippet: The in vitro confrontation test was performed in 90 mm Petri dishes on 1.5% malt agar supplemented or not with 50 μg/mL chloramphenicol (Merck, Sigma Aldrich) and 50 μg/mL streptomycin sulfate (Merck, Sigma Aldrich). .. To sequence the V3-V4 region of the bacterial 16S rRNA gene, two-step, Nextera barcoded PCR libraries were generated using 341 F (5′- CCT ACG GGN GGC WGC AG -3′) and 805 R (5′- GAC TAC HVG GGT ATC TAA TCC -3′) with 20 PCR cycles for the first step and 10 PCR cycles for the second step. .. To sequence the internal transcribed spacer (ITS2) regions of the fungal 18S rRNA gene, two-step, Nextera barcoded PCR libraries were generated using ITS3 (5′- GCA TCG ATG AAG AAC GCA GC -3′), and ITS4 (5′- TCC TCC GCT TAT TGA TAT GC -3′) with 20 PCR cycles for the first step and 15 PCR cycles for the second step.

    Polymerase Chain Reaction:

    Article Title: Microbial bioremediation of persistent organic pollutants in plant tissues provides crop growth promoting liquid fertilizer.
    Article Snippet: The concentration of the isolated DNA was assessed via PicoGreen measurement (Quant-iTTM PicoGreenTM dsDNA Assay Kit, Thermo Fisher). .. To sequence theV3-V4 regionof thebacterial 16S rRNAgene, two-step, Nextera barcoded PCR libraries were generated using 341 F (5′- CCT ACGGGNGGCWGCAG -3′) and 805R (5′- GACTACHVGGGTATCTAA TCC -3′) with 20 PCR cycles for the first step and 10 PCR cycles for the second step. .. To sequence the internal transcribed spacer (ITS2) regions of the fungal 18S rRNA gene, two-step, Nextera barcoded PCR libraries were generated using ITS3 (5′- GCA TCG ATG AAG AAC GCA GC -3′), and ITS4 (5′- TCC TCC GCT TAT TGA TAT GC -3′) with 20 PCR cycles for the first step and 15 PCR cycles for the second step.

    Article Title: eDNA metabarcoding reveals high soil fungal diversity and variation in community composition among Spanish cliffs
    Article Snippet: The concentration of the isolated DNA was assessed with PicoGreen measurement (Quant‐iTTM PicoGreenTM dsDNA Assay Kit; Thermo Fisher). .. To sequence the internal transcribed spacer (ITS2) regions of the fungal 18 S rRNA gene, two‐step, Nextera barcoded PCR libraries using the locus‐specific primer pair ITS3 (5′‐GCA TCG ATG AAG AAC GCA GC‐3′) and ITS4 (5′‐TCC TCC GCT TAT TGA TAT GC‐3′) with 20 PCR cycles for the first step and 20 PCR cycles for the second step were created. .. Subsequently, the PCR libraries were sequenced on an Illumina MiSeq platform using a v2 500 cycles kit.

    Article Title: Drivers of wood‐inhabiting fungal diversity in European and Oriental beech forests
    Article Snippet: DNA concentrations were quantified using a NanoDrop UV–Vis spectrophotometer (Peqlab Biotechnologie GmbH, Erlangen, Germany). .. For sequencing the internal transcribed spacer (ITS2) regions of the fungal 18S rRNA gene, we applied for two‐step, Nextera barcoded PCR libraries using the locus specific primer pair ITS3 (5′‐GCA TCG ATG AAG AAC GCA GC‐3′) and ITS4 (5′‐TCC TCC GCT TAT TGA TAT GC‐3′) with 20 PCR cycles for the first step and 15 PCR cycles for the second step were created. .. Subsequently the PCR libraries were sequenced on an Illumina MiSeq platform using a v2 500 cycles kit.

    Article Title: Microbial bioremediation of persistent organic pollutants in plant tissues provides crop growth promoting liquid fertilizer.
    Article Snippet: To sequence theV3-V4 regionof thebacterial 16S rRNAgene, two-step, Nextera barcoded PCR libraries were generated using 341 F (5′- CCT ACGGGNGGCWGCAG -3′) and 805R (5′- GACTACHVGGGTATCTAA TCC -3′) with 20 PCR cycles for the first step and 10 PCR cycles for the second step. .. To sequence the internal transcribed spacer (ITS2) regions of the fungal 18S rRNA gene, two-step, Nextera barcoded PCR libraries were generated using ITS3 (5′- GCA TCG ATG AAG AAC GCA GC -3′), and ITS4 (5′- TCC TCC GCT TAT TGA TAT GC -3′) with 20 PCR cycles for the first step and 15 PCR cycles for the second step. ..

    Article Title: Microbial bioremediation of persistent organic pollutants in plant tissues provides crop growth promoting liquid fertilizer
    Article Snippet: The in vitro confrontation test was performed in 90 mm Petri dishes on 1.5% malt agar supplemented or not with 50 μg/mL chloramphenicol (Merck, Sigma Aldrich) and 50 μg/mL streptomycin sulfate (Merck, Sigma Aldrich). .. To sequence the V3-V4 region of the bacterial 16S rRNA gene, two-step, Nextera barcoded PCR libraries were generated using 341 F (5′- CCT ACG GGN GGC WGC AG -3′) and 805 R (5′- GAC TAC HVG GGT ATC TAA TCC -3′) with 20 PCR cycles for the first step and 10 PCR cycles for the second step. .. To sequence the internal transcribed spacer (ITS2) regions of the fungal 18S rRNA gene, two-step, Nextera barcoded PCR libraries were generated using ITS3 (5′- GCA TCG ATG AAG AAC GCA GC -3′), and ITS4 (5′- TCC TCC GCT TAT TGA TAT GC -3′) with 20 PCR cycles for the first step and 15 PCR cycles for the second step.

    Generated:

    Article Title: Microbial bioremediation of persistent organic pollutants in plant tissues provides crop growth promoting liquid fertilizer.
    Article Snippet: The concentration of the isolated DNA was assessed via PicoGreen measurement (Quant-iTTM PicoGreenTM dsDNA Assay Kit, Thermo Fisher). .. To sequence theV3-V4 regionof thebacterial 16S rRNAgene, two-step, Nextera barcoded PCR libraries were generated using 341 F (5′- CCT ACGGGNGGCWGCAG -3′) and 805R (5′- GACTACHVGGGTATCTAA TCC -3′) with 20 PCR cycles for the first step and 10 PCR cycles for the second step. .. To sequence the internal transcribed spacer (ITS2) regions of the fungal 18S rRNA gene, two-step, Nextera barcoded PCR libraries were generated using ITS3 (5′- GCA TCG ATG AAG AAC GCA GC -3′), and ITS4 (5′- TCC TCC GCT TAT TGA TAT GC -3′) with 20 PCR cycles for the first step and 15 PCR cycles for the second step.

    Article Title: Microbial bioremediation of persistent organic pollutants in plant tissues provides crop growth promoting liquid fertilizer.
    Article Snippet: To sequence theV3-V4 regionof thebacterial 16S rRNAgene, two-step, Nextera barcoded PCR libraries were generated using 341 F (5′- CCT ACGGGNGGCWGCAG -3′) and 805R (5′- GACTACHVGGGTATCTAA TCC -3′) with 20 PCR cycles for the first step and 10 PCR cycles for the second step. .. To sequence the internal transcribed spacer (ITS2) regions of the fungal 18S rRNA gene, two-step, Nextera barcoded PCR libraries were generated using ITS3 (5′- GCA TCG ATG AAG AAC GCA GC -3′), and ITS4 (5′- TCC TCC GCT TAT TGA TAT GC -3′) with 20 PCR cycles for the first step and 15 PCR cycles for the second step. ..

    Article Title: Microbial bioremediation of persistent organic pollutants in plant tissues provides crop growth promoting liquid fertilizer
    Article Snippet: The in vitro confrontation test was performed in 90 mm Petri dishes on 1.5% malt agar supplemented or not with 50 μg/mL chloramphenicol (Merck, Sigma Aldrich) and 50 μg/mL streptomycin sulfate (Merck, Sigma Aldrich). .. To sequence the V3-V4 region of the bacterial 16S rRNA gene, two-step, Nextera barcoded PCR libraries were generated using 341 F (5′- CCT ACG GGN GGC WGC AG -3′) and 805 R (5′- GAC TAC HVG GGT ATC TAA TCC -3′) with 20 PCR cycles for the first step and 10 PCR cycles for the second step. .. To sequence the internal transcribed spacer (ITS2) regions of the fungal 18S rRNA gene, two-step, Nextera barcoded PCR libraries were generated using ITS3 (5′- GCA TCG ATG AAG AAC GCA GC -3′), and ITS4 (5′- TCC TCC GCT TAT TGA TAT GC -3′) with 20 PCR cycles for the first step and 15 PCR cycles for the second step.



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